Application

Research Sub CategoryFragment Specific Secondary Antibodies

Rabbit anti-Goat IgG Antibody, Fc, HRP conjugate is an antibody against Goat IgG for use in ELISA & WB.

Research CategorySecondary & Control Antibodies

ELISA and Western Blotting 1:5,000 - 1:100,000

Immunohistochemistry 1:500 - 1:5,000

Optimal working dilutions must be determined by the end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Lyophilized. Buffer = 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6, with 15mg/mL BSA and 0.01% Thimerosal.

RECONSTITUTION:

Reconstitute with sterile distilled water to match the volume indicated on the label. Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature.

Specificity

Based on immunoelectrophoresis, the antibody reacts with the heavy chains on goat IgG but not with the light chains on most goat immunoglobulins. No antibody was detected against goat IgM, or against immunoglobulin serum proteins, but antibodies may cross-react with immunoglobulins from other species.

Storage and Stability

Maintain lyophilized product at 2-8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. After dilution do not use for more than one day. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.

WARNING:

Use of sodium azide as a preservative will substantially inhibit the enzyme activity of HRP.